Members of the glial cell line-derived neurotropic factor (GDNF) family,including GDNF and neuturin (NTN),play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNR,which is a cell surface receptor for NTN. The cDNA encoding NTNR from human,rat,chicken and mouse have been cloned recently. NTNR was also termed GDNFRB Ret ligand 2 (RETL2),or TGF-B-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFR-2 recently. GFR-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus,NTN,GFR-2 and the Ret PTK from a complex to transduce NTN signal and to mediate NTN function.
应用类型
Western blot analysis (1 ug/ml) and a 52 kDa band should be detected. However, the optimal conditions should be determined individually.